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natK [2019-09-02 12:16:01]
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natK [2019-09-02 12:16:01]

two-component sensor kinase, regulation of the [gene|B019FD0CB6F76C03DA5E76ABD1B324BA5E1A6936|natA]-[gene|A8ADA02BBB549866DE330040CF6D06ECC3B00099|natB] operon
locus
BSU_02730
pI
8.05
mw
34.92 kDa
protein length
318 aa Sequence Blast
gene length
957 bp Sequence Blast
function
regulation of the [gene|B019FD0CB6F76C03DA5E76ABD1B324BA5E1A6936|natA]-[gene|A8ADA02BBB549866DE330040CF6D06ECC3B00099|natB] operon
product
two-component sensor kinase
essential
no
synonyms
yccG
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.1|Two-component sensor kinase]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

    • Gene

    Coordinates
    294,615 295,571

    The protein

    Catalyzed reaction/ biological activity

  • autophosphorylation, phosphorylation of [protein|D3F6337B94A633730E514B05CA852BE9AA3741B3|NatR] in response to sodium availability
  • ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)

    • [SW|Domains]

  • three transmembrane segments, C-terminal histidine phosphotransferase domain
  • [SW|Histidine kinase domain] (aa 132-318) (according to UniProt)

    • Modification

  • autophosphorylation on a His residue

    • [SW|Localization]

  • cell membrane (according to UniProt)

    • Expression and Regulation

    Operons

    genes
    [gene|D3F6337B94A633730E514B05CA852BE9AA3741B3|natR]-[gene|A4644CB18F7D0DF3E7E50ABB0C0F224D5744A1A5|natK]
    description
    [pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-C042 (yccG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2040 NBRP B. subtilis, Japan]
  • BKE02730 ([gene|A4644CB18F7D0DF3E7E50ABB0C0F224D5744A1A5|natK]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE02730 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AAGGGTAATCACGTACTAAA, downstream forward: _UP4_TAAAAAAACCTTGAAAAGCC
  • BKK02730 ([gene|A4644CB18F7D0DF3E7E50ABB0C0F224D5744A1A5|natK]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK02730 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AAGGGTAATCACGTACTAAA, downstream forward: _UP4_TAAAAAAACCTTGAAAAGCC

    • References

  • 10094672,17322186